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Energy and Biotechnology Lab

Members

Ph.D. Student



[Integrated Ph.D.] Basit Sharif
  • Mailbasit.sharif99@gm.gist.ac.kr

Education

  • B. S. Department of Electrical Engineering, International Islamic University (IIU) Islamabad, Pakistan

Publication

  • Reginald, S.S., Lee, H., Fazil, N., Sharif, B., Lee, M., Kim, M.J., Haluk, B. Chang, I.S. (2022) Control of Carbon Monoxide Dehydrogenase Orientation by Site-Specific Immobilization Enables Direct Electrical Contact Between Enzyme Cofactor and Solid Surface. Communications Biology, 5(390).

Research Interests

  • Bioelectrical systems
  • Enzyme/protein engineering


[Ph.D.] 강병찬 (Byeongchan Kang)
  • Mailkangbc15107@gm.gist.ac.kr

Education

  • M. S. School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Korea
  • B. S. Dept. of Biotechnology, Chonnam National University, Korea

Thesis for M.S. degree

  • Identification of CODH Transcription Regulator in Eubacterium limosum KIST612 using GUS Reporter System

Publication

  • Kim, J.-Y.*, Park, S.*, Jeong, J., Lee, M., Kang, B., Jang, S.H., Jeon, J., Jang, N., Oh, S., Park, Z.-Y., Chang, I.S., (2021) Methanol supply speeds up synthesis gas fermentation by methylotrophic-acetogenic bacterium, Eubacterium limosum KIST612, Bioresource Technology, 321, 124521.

Research Interests

  • Metabolic / genetic engineering


[Ph.D.] 오소영 (Soyoung Oh)
  • Mailsysy0418@gm.gist.ac.kr

Education

  • M. S. School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Korea
  • B. S. Department of Energy and Resources Engineering, Chonnam National University, Korea

Thesis for M.S. degree

  • Metabolic Engineering of Eubacterium limosum KIST612 for 2,3-butanediol Production

Publication

  • Reginald, S.S., Kim, M.J., Lee, H., Fazil, N., Choi, S., Oh, S., Seo, J., Chang, I.S. (2022) Direct Electrical Contact of NAD+/NADH-Dependent Dehydrogenase on Electrode Surface Enabled by Non-Native Solid-Binding Peptide as a Molecular Binder. Electrochimica Acta, 421, pp. 140480.
  • Kang, H.*, Park, B.*, Oh, S.*, Pathiraja, D., Kim, J.-Y., Jung, S., Jeong, J., Cha, M., Park, Z.-Y., Choi, I.G., Chang, I.S. (2021) Metabolism Perturbation Caused by the Overexpression of Carbon Monoxide Dehydrogenase/Acetyl-CoA Synthase Gene Complex Accelerated Gas to Acetate Conversion Rate of Eubacterium limosum KIST612. Bioresource Technology, 125879.
  • Kim, J.-Y.*, Park, S.*, Jeong, J., Lee, M., Kang, B., Jang, S.H., Jeon, J., Jang, N., Oh, S., Park, Z.-Y., Chang, I.S., (2021) Methanol supply speeds up synthesis gas fermentation by methylotrophic-acetogenic bacterium, Eubacterium limosum KIST612, Bioresource Technology, 321, 124521.

Research Interests

  • Metabolic / genetic engineering



[Ph.D.] 김지연 (Ji-Yeon Kim)
  • Mailjiyeonkim@gm.gist.ac.kr

Education

  • M. S. School of Applied Biosciences, Kyungpook National University, Korea
  • B. S. School of Applied Biosciences, Kyungpook National University, Korea

Thesis for M.S. degree

  • Physiological and Transcriptomic Analysis of the Native-feather Degrading Extremophilic Anaerobe Fervidobacterium islandicum AW-1

Publication

  • Kang, H.*, Park, B.*, Oh, S.*, Pathiraja, D., Kim, J.-Y., Jung, S., Jeong, J., Cha, M., Park, Z.-Y., Choi, I.G., Chang, I.S. (2021) Metabolism Perturbation Caused by the Overexpression of Carbon Monoxide Dehydrogenase/Acetyl-CoA Synthase Gene Complex Accelerated Gas to Acetate Conversion Rate of Eubacterium limosum KIST612. Bioresource Technology, 125879.
  • Kim, J.-Y.*, Park, S.*, Jeong, J., Lee, M., Kang, B., Jang, S.H., Jeon, J., Jang, N., Oh, S., Park, Z.-Y., Chang, I.S., (2021) Methanol supply speeds up synthesis gas fermentation by methylotrophic-acetogenic bacterium, Eubacterium limosum KIST612, Bioresource Technology, 321, 124521.
  • J. Jeong*, J.-Y. Kim*, B. Park, I.-G. Choi, I. S. Chang, (2020) Genetic engineering system for syngas-utilizing acetogen, Eubacterium limosum KIST612. Bioresource Technology Reports, 11: 100452.

Research Interests

  • Metabolic engineering



[Ph.D.] 이문규 (Mungyu Lee)
  • Mailspdetective@gm.gist.ac.kr

Education

  • M. S. Department of Environmental Science and Engineering, Kyung Hee University, Korea
  • B. S. Department of Environmental Science and Engineering, Kyung Hee University, Korea

Thesis for M.S. degree

  • Integration of microbial electrosynthesis to anaerobic digestion at different glucose concentrations

Publication

  • Reginald, S.S., Lee, H., Fazil, N., Sharif, B., Lee, M., Kim, M.J., Haluk, B. Chang, I.S. (2022) Control of Carbon Monoxide Dehydrogenase Orientation by Site-Specific Immobilization Enables Direct Electrical Contact Between Enzyme Cofactor and Solid Surface. Communications Biology, 5(390).
  • Kim, J.-Y.*, Park, S.*, Jeong, J., Lee, M., Kang, B., Jang, S.H., Jeon, J., Jang, N., Oh, S., Park, Z.-Y., Chang, I.S., (2021) Methanol Supply Speeds Up Synthesis Gas Fermentation by Methylotrophic-Acetogenic Bacterium, Eubacterium limosum KIST612, Bioresource Technology, 321, 124521.
  • Kim, B., Jang, N., Lee, M., Chang, I.S. (2021) Microbial Fuel Cell Driven Mineral Rich Wastewater Treatment Process for Circular Economy by Creating Virtuous Cycles. Bioresource Technology, 320, 124254.

Research Interests

  • Gas-Liquid Mass Transfer in Bioreactor System
  • Bioreactor operation and optimization



[Ph.D.] 이혜령 (Hyeryeong Lee)
  • Mailheather@gm.gist.ac.kr

Education

  • M. S. School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Korea
  • B. S. Dept. of Applied Chemical Engineering, Chonnam National University, Korea

Thesis for M.S. degree

  • Significance of Inter-Enzyme Effect on Direct Electron Transfer of Glucose Dehydrogenase Immobilized Electrode

Publication

  • Reginald, S.S., Kim, M.J., Lee, H., Fazil, N., Choi, S., Oh, S., Seo, J., Chang, I.S. (2022) Direct Electrical Contact of NAD+/NADH-Dependent Dehydrogenase on Electrode Surface Enabled by Non-Native Solid-Binding Peptide as a Molecular Binder. Electrochimica Acta, 421, pp. 140480.
  • Reginald, S.S., Lee, H., Fazil, N., Sharif, B., Lee, M., Kim, M.J., Haluk, B. Chang, I.S. (2022) Control of Carbon Monoxide Dehydrogenase Orientation by Site-Specific Immobilization Enables Direct Electrical Contact Between Enzyme Cofactor and Solid Surface. Communications Biology, 5(390).
  • Lee, H., Lee, E.M., Reginald, S.S., Chang, I.S. (2021) Peptide Sequence-Driven Direct Electron Transfer Properties and Binding Behaviors of Gold Binding Peptide-Fused Glucose Dehydrogenase on Electrode. iScience.
  • Lee, H., Lee, Y.S., Resinald, S.S., Baek, S., Lee, E.M., Choi, I.-G., Chang, I.S. (2020) Biosensing and electrochemical properties of flavin adenine dinucleotide (FAD)-Dependent glucose dehydrogenase (GDH) fused to a gold binding peptide. Biosens. Bioelectron. 165,112427
  • Reginald, S.S*., Lee, H*., Lee, Y.S., Yasin, M., Chang, I.S. (2020) Dissolved carbon monoxide concentration monitoring platform based on direct electrical connection of CO dehydrogenase with electrically accessible surface structure. Bioresource Technology, 297
  • Reginald, S.S., Lee, Y.S., Lee, H., Jang, N., Chang, I.S. (2019) Electrocatalytic and Biosensing Properties of Aerobic Carbon Monoxide Dehydrogenase from Hydrogenophaga Pseudoflava Immobilized on Au Electrode towards Carbon Monoxide Oxidation. Electroanalysis, 31 (9), pp. 1635-1640.
  • Lee, H., Lee, Y.S., Lee, S.K., Baek, S., Choi, I.-G., Jang, J.H., Chang, I.S. (2019) Significant enhancement of direct electric communication across enzyme-electrode interface via nano-patterning of synthetic glucose dehydrogenase on spatially tunable gold nanoparticle (AuNP)-modified electrode. Biosensors and Bioelectronics, 126, pp. 170-177.
  • Lee, Y.S., Baek, S., Lee, H., Reginald, S.S., Kim, Y., Kang, H., Choi, I.-G., Chang, I.S. (2018) Construction of Uniform Monolayer- and Orientation-Tunable Enzyme Electrode by a Synthetic Glucose Dehydrogenase without Electron-Transfer Subunit via Optimized Site-Specific Gold-Binding Peptide Capable of Direct Electron Transfer. ACS Applied Materials and Interfaces, 10 (34), pp. 28615-28626.
  • Kim, T., An, J., Lee, H., Jang, J.K., Chang, I.S. (2016) pH-dependent ammonia removal pathways in microbial fuel cell system. Bioresource Technology, 215, pp. 290-295.

Research Interests

  • Protein-based bioelectrochemistry
  • Protein/inorganic interface



[Integrated Ph.D.] 최세라 (Serah Choi)
  • Mailserahchoi22@gist.ac.kr

Education

  • B. S. Dept. of Biological Chemistry Engineering, Dongguk University, Korea

Publication

  • Reginald, S.S., Kim, M.J., Lee, H., Fazil, N., Choi, S., Oh, S., Seo, J., Chang, I.S. (2022) Direct Electrical Contact of NAD+/NADH-Dependent Dehydrogenase on Electrode Surface Enabled by Non-Native Solid-Binding Peptide as a Molecular Binder. Electrochimica Acta, 421, pp. 140480.
  • Choi, S., Kim, B., Chang, I.S. (2018) Tracking of Shewanella oneidensis MR-1 biofilm formation of a microbial electrochemical system via differential pulse voltammetry. Bioresource Technology, 254, pp. 357-361.
  • Kim, B., Choi, S., Jang, J.K., Chang, I.S. (2017) Self-recoverable voltage reversal in stacked microbial fuel cells due to biofilm capacitance. Bioresource Technology, 245, pp. 1286-1289.

Research Interests

  • Extracellular electron transfer mechanism, Biofilm, Microbial electrochemical system
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